TOJSAT - Volume 10 - Issue 2 - April 2020
EXPRESSION OF EPIDERMAL GROWTH FACTOR RECEPTOR AFTER LOW DOSE CAPSAICIN ADMINISTRATION IN RAT DUODENUM Ender Deniz ASMAZ, Berrin ZIK
Abstract:
Growth factors are essential for the development, growth and homeostasis of
multicellular organisms and play an important role in the development and the maintenance of
the gastrointestinal tract. Capsaicin (CAP) with neurotoxic properties has been shown to have
a protective effect against experimental gastric mucosal injury in animals and humans.
Epidermal growth factor (EGF) and ıt’s receptore has been shown to exert gastric hyperemic
and gastroprotective effects via capsaicin-sensitive afferent neurons, including the release of
calcitonin gene-related peptide (CGRP). The. aim of the present study was to investigate the
effects of low dose capsaicin (CAP) on epidermal growth factor receptor (EGFR) expression in
the duodenum. In this study, 21-day-old rats were divided into two groups as CAP-treated and
vehicle. CAP prepared in a solvent and injected subcutaneously to CAP-treated group (0.5
mg/kg/d) and vehicle group was injected with only solvent for 20 days. At the end of the
experiment, tissue samples were collected and paraffin-embedded tissues were processed for
standard immunohistochemistry by the labelled streptavidin-biotin technique. The EGFR
localizations were identified on the surface epithelium of the villi, Lieberkühn crypts, in the
Brunner’s glands and smooth muscles layer of the duodenumIn the experimental group, the
expression of EGFR in surface epithelial cells was not different compared to the control group,
while the expressions in the Lieberukhn crypts, Brunner's glands and smooth muscle layer were
stronger than the control group. As a result, the low dose capsaicin increased EGFR expression
in the digestive system and probably have positive effects on the digestive system
GENERATION OF CELLULAR CLONES FOR APOBEC1 ENZYME AND AUXILIARY COFACTORS Mahmut ÖZER, Veysel TOLAN
Abstract:
RNA editing is defined as any site-specific alteration in RNA sequence excluding
the post-transcriptional modifications namely 5’ 7-methylguanosine capping, 3’
polyadenylation and pre-mRNA splicing. Almost all of the RNA editing processes so far
describe the changes in mRNA sequences which result in the production of altered protein
products. In this study, generation of some cellular clones for the analysis of APOBEC1
(apolipoprotein B mRNA editing enzyme, catalytic polypeptide)-induced RNA editing has been
aimed. In one hand, conditions for the cytodifferentiation of Caco-2 cells to allow them to
resemble phenotypically the small intestine enterocytes where the mRNA editing of
apolipoprotein B takes place in vivo has been set. On the other hand, inactivation of the genes
that code for APOBEC1, ACF (APOBEC-1 Complementation Factor) and RBM47 (RNA
Binding Motif47) has been performed. To achieve this, a very recent and popular genome
editing tool derived from the bacterial CRISPR (Clustered Regularly Interspaced Short
Palindromic Repeats) / Cas9 (CRISPR-associated 9) system was utilized.
READING THE STREETS OF BALAT AND URBAN IDENTITY Serdar Kasap, Sezer Yörük
Abstract:
Being one of the biggest metropolises in the world, Ä°stanbul is a peerless city with
its natural beauties, history, archaeological heritage and socio-cultural life. These properties
both create the rich identity of the city and provide a strong urban image. The skyline composed
of the historical texture of the city and physical elements offers an extremely impressive view.
Fener-Balat is an important element of the physical structure of the city of Istanbul. It plays an
important role for historic and cultural values of Istanbul to take a shape. As an unforgettable
part of the metropolis of Istanbul, Balat where Turkish, Rum, Armenian, Jewish and Bulgarian
identities and mosques, churches and synagogues co-exist has the characteristic of a sub-region
with strong elements forming its identity. It is observed that big changes have occurred in social
textures and population compositions of these regions. The innermost parts of the city, where
particularly the urbanized, settled upper-middle class families have left became the areas where
poor or immigrant population became dense. The world continuously keeps changing within
the frame of socio-cultural, physical, economical, political and technological dynamics and this
change reflects on urban areas. The urban area which takes shape as a result of mutual
interaction of these dynamics involves some differences and similarities in every age, every
country and every city. Therefore no city is being shaped in the same way as others.
In the study, the relationships established by various social groups with the region are examined
through printed texts and news on Internet. All sources are compared in the study and analyses
of language, wording and ideological approaches are made and the meaning of Fener-Balat area
for different groups is discussed. It s observed that the memory generated in the news on Internet
regarding Fener-Balat region is composed of images generated by the groups from their
ideological positions. In printed sources, on the other hand, it is observed that some kind of a
popular history is narrated rather than generating a memory.
STUDY ON SHUNT CURRENTS IN A MULTI-STACK VANADIUM FLOW BATTERY SYSTEM Han-Wen Chou, Hwa-Jou Wei, Feng-Zhi Chang, Amornchai Arpornwichanop, Yong-Song Chen
Abstract:
An all-vanadium redox flow battery (VRFB) is an energy storage device that uses
the redox reaction of vanadium ions with different oxidation states. It has the advantages of
quick conversion between charge and discharge processes, long operation life, separate power
and energy capacity designs; as a result, it has been considered as one of potential candidates
as energy storage systems for renewable energies. Because the electrolyte is electrically
conductive, shunt currents occur within a multi-cell stack and within the piping system that
connecting stacks. Shunt currents are affected by cell number in a single stack, stack number,
dimensions of flow channels. In this study, a mathematical model is developed to determine
shunt currents in a multi-stack system. The effect of stack number on charge transfer efficiency
are predicted by this model. Results show that central stacks in a VRFB system provide more
current. The maximum cell current decreases with increasing stack number.
THE EFFICACY OF THE HERBAL IODINE SOLUTION CONCENTRATE FROM THE BRAND OF "JADRANKINA OTOPINA" (JADRANKA'S SOLUTION) IN THE TREATMENT OF THYROID DISEASES Gordana Bujisic, Slavica Jankovic, Erna Davidovic Cvetko, Ilja Uzelac Bujisic
Abstract:
This paper presents the results of the research on effectiveness of the Herbal Iodine
Solution Concentrate “Jadranka's Solution” on diseases of thyroid gland. The research included
64 participants who fulfilled detailed online questionnaire about their socio-demographic data
and experiences they had with the Herbal Iodine Solution Concentrate „Jadranka's Solution“.
Along with that, each participant sent us two laboratory findings of the blood’ level of TSH and
T4. The most important result of the research was confirmation of the hypothesis that regular
use of the Herbal Iodine Solution Concentrate “Jadranka's Solution” improves clinical status of
patients with disorders of thyroid gland. Pairwise' comparison has confirmed significant
difference at the ,05 level for levels of TSH and T4 before and after the use of “Jadranka
Solution”. Statistically, the results of the study showed a significant decrease of the values of
TSH, and an increase in FT3 and FT4 hormones to their mid-values and above the reference
value, when comparing values at the point of diagnosed health issues.
These results confirms subjective evaluation of the participants who, in 98.5% of participants
evaluated effectiveness of Herbal Iodine Solution Concentrate “Jadranka's Solution” as
excellent and none of them evaluated Solution as ineffective. Speaking about sociodemographic
data, most of the participants were 30 to 40 years old (56%), 49,2% of them have
university' degree and 68.6% of them is employed. These data point that participants are better
educated and informed than overage population in Croatia. Most of them use Herbal Iodine
Solution Concentrate “Jadranka's Solution” because of diseases of thyroid gland, 73.4%
because of hypothyroidism and 7.8% because of hyperthyroidism.
THE USE OF A SENSOR NETWORK IN THE PROMOTION OF THE HEALTH OF THE DEPENDENT ELDERLY AT HOME Vitor Rodrigues, Maria João Monteiro, Salviano Soares, António Valente, Sérgio Silva, Madalena Sousa, Diogo Duarte, Conceição Rainho, Isabel Barroso, Carlos Almeida, João Castro, Amâncio Carvalho, Cristina Antunes, Filomena Raimundo
Abstract:
The aging population and the increasing longevity of individuals is a challenging
reality for today’s healthcare, as it brings increased dependency and need for continuous care,
which is often left to informal caregivers. This will be an “almost experimental” study as the
investigators will attempt to control some independent variables through the implementation of
a therapeutic plan’s assistance and vigilance program from a sensor network, which will
monitor and assist (alarm sending) in complying with medication, raisings and positionings.
28.6% of the elderly cannot move alone in bed and 100% of the elderly take medication
regularly. In the control group, there are 6 elderly people with total dependence, 3 moderately
dependent elderly and 5 elderly with slight dependence. So, knowing that Portugal has a high
rate of informal home care, it was relevant to develop this project for the application of
information and communication technologies in the development of a prototype of a system
focused on the monitoring and aid of the execution of the therapeutic plan in two strands,
mobilization and medication.
